Ni Agarose for electrophoresis,Agarose chemical reagent

Agarose is a quality general application agarose that provides good resolution and is cost effective for high volume users

In general, Ni2+is the preferred metal ion for purification of histidine-tagged proteins. The IMAC medium Ni Sepharose CL-4B consists of 90 µm beads of highly cross-linked agarose, to which a chelating group has been coupled. This chelating group has then been charged with nickel (Ni2+) ions.Ni Sepharose CL-4B has low Ni2+leakage, high protein-binding capacity, and is compatible with a wide range of additives used in protein purification. Its high flow properties make it excellent for scale-up. The medium is also suitable for gravity flow column purification and multiwell plate screening.

Medium characteristics

Matrix	Highly cross-linked spherical agarose, 4%
Dynamic binding capacity*	Approx. 40 mg (histidine)6-tagged protein/ml medium
Metal ion capacity	Approx. 15 µmol Ni2+/ml medium
Average particle size	90 µm
Recommended flow rate†	<150 cm/h
Max. operating pressure†	0.1 MPa, 1 bar (when packed in XK columns.  May vary if used in other columns)
Chemical stability‡	Stable in: 0.01 M HCl, 0.1 M NaOH. Tested for 1 week at 40°C.  1 M NaOH, 70% acetic acid. Tested for 12 hours.   2% SDS. Tested for 1 hour.  30% 2-propanol. Tested for 30 min.
pH stability‡	Short term (at least 2 hours) 2–14   Long term (≤1 week) 3–12
Storage	20% ethanol
Storage temperature	4°C to 30°C

Ni Agarose for electrophoresis,Agarose chemical reagent