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Ni Agarose for electrophoresis,Agarose chemical reagent

Ni Agarose for electrophoresis,Agarose chemical reagent
Product Detailed
Related Categories:Chemical Reagents
Agarose is a quality general application agarose that provides good resolution and is cost effective for high volume users

In general, Ni2+is the preferred metal ion for purification of histidine-tagged proteins. The IMAC medium Ni Sepharose CL-4B consists of 90 µm beads of highly cross-linked agarose, to which a chelating group has been coupled. This chelating group has then been charged with nickel (Ni2+) ions.Ni Sepharose CL-4B has low Ni2+leakage, high protein-binding capacity, and is compatible with a wide range of additives used in protein purification. Its high flow properties make it excellent for scale-up. The medium is also suitable for gravity flow column purification and multiwell plate screening.

Medium characteristics

Matrix Highly cross-linked spherical agarose, 4%
 Dynamic binding capacity* Approx. 40 mg (histidine)6-tagged protein/ml medium
 Metal ion capacity 

 Approx. 15 µmol Ni2+/ml medium

 Average particle size 

90 µm

 Recommended flow rate† 

 <150 cm/h

 Max. operating pressure†  

0.1 MPa, 1 bar (when packed in XK columns.

 May vary if used in other columns)

 Chemical stability‡

  Stable in: 0.01 M HCl, 0.1 M NaOH. Tested for 1 week at 40°C.

 1 M NaOH, 70% acetic acid. Tested for 12 hours.

  2% SDS. Tested for 1 hour.

 30% 2-propanol. Tested for 30 min.

 pH stability‡  

Short term (at least 2 hours) 2–14

  Long term (≤1 week) 3–12

 Storage  20% ethanol
 Storage temperature 

 4°C to 30°C

Ni Agarose for electrophoresis,Agarose chemical reagent



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