HS Taq DNA Polymerase,PCR amplification Polymerase

HS Taq DNA Polymerase 1,Rapid PCR amplification 2,Efficient PCR amplification 3,Good heat resistance

Description

    HS Taq DNA Polymerase is derived from the Thermu aquaticus DNA Polymerase gene screening by DNA shuffling to obtain mutants, for Hot Start PCR.Enzyme at room temperature or 37 °C, activity was inhibited, thus inhibiting conditions caused by low non-specific annealing or primer-dimers caused by non-specific amplification, and high temperature (68 °C above) enzyme activity is activated.Mutations induced by recombinant E. coli, after obtained the two separate purified, high purity. PCR products amplified 3 'end with the "A" bases can be directly used for TA cloning.

PCR performance

  1,With Lambda DNA as a template, can be a good amplification of DNA fragments 8kb

  2,Human genomic DNA as a template, can be a good 2.3 kb fragment was amplified

  3,55 °C reaction conditions 10min, the activity of Taq polymerase inhibition efficiency of 90%

Rapid PCR amplification

1kb DNA fragment was amplified, only 35 min

Efficient PCR amplification

By hot start PCR, to improve the performance of PCR, to achieve a high specificity of the PCR reaction.

Good heat resistance

                                         

HS Taq DNA Polymerase,PCR amplification Polymerase